2XWU
CRYSTAL STRUCTURE OF IMPORTIN 13 - UBC9 COMPLEX
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-07-27 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 68.700, 126.800, 184.000 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.170 - 2.800 |
| R-factor | 0.226 |
| Rwork | 0.226 |
| R-free | 0.26800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB ENTRIES 2X19 AND 1U9A |
| RMSD bond length | 0.010 |
| RMSD bond angle | 0.405 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | CNS (1.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.900 |
| High resolution limit [Å] | 2.800 | 1.800 |
| Rmerge | 0.070 | 0.680 |
| Number of reflections | 40287 | |
| <I/σ(I)> | 14.49 | 2.37 |
| Completeness [%] | 99.4 | 99.9 |
| Redundancy | 3.7 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 50 MM MES PH 6.5 25% PEG 300 |
