2XWS
ANAEROBIC COBALT CHELATASE (CbiX) FROM ARCHAEOGLOBUS FULGIDUS
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC CCD |
| Spacegroup name | P 42 21 2 |
| Unit cell lengths | 50.980, 50.980, 101.970 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 33.990 - 1.600 |
| R-factor | 0.18679 |
| Rwork | 0.185 |
| R-free | 0.21484 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2dj5 |
| RMSD bond length | 0.029 |
| RMSD bond angle | 2.402 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.500 | 1.690 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.050 | 0.130 |
| Number of reflections | 118464 | |
| <I/σ(I)> | 23.6 | 13.3 |
| Completeness [%] | 99.4 | 100 |
| Redundancy | 6.4 | 6.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.8 | pH 6.8 |
