2XW0
Human serum albumin complexed with dansyl-L-phenylalanine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX14.1 |
| Synchrotron site | SRS |
| Beamline | PX14.1 |
| Temperature [K] | 298 |
| Detector technology | CCD |
| Collection date | 2007-07-25 |
| Detector | ADSC CCD |
| Spacegroup name | P 1 |
| Unit cell lengths | 55.172, 55.429, 119.937 |
| Unit cell angles | 81.12, 91.04, 64.78 |
Refinement procedure
| Resolution | 28.910 - 2.400 |
| R-factor | 0.225 |
| Rwork | 0.225 |
| R-free | 0.25400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2bxa |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.200 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | CNS |
| Refinement software | CNS (1.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.500 | 2.550 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.080 | 0.380 |
| Number of reflections | 49448 | |
| <I/σ(I)> | 8.3 | 1.4 |
| Completeness [%] | 88.0 | 78.2 |
| Redundancy | 1.8 | 1.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | SEE PAPER, pH 7 |
