2XVT

Structure of the extracellular domain of human RAMP2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I02
Synchrotron site	Diamond
Beamline	I02
Temperature [K]	100
Detector technology	CCD
Collection date	2010-08-11
Detector	ADSC CCD
Spacegroup name	P 21 21 21
Unit cell lengths	59.759, 90.984, 92.056
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	64.710 - 2.050
R-factor	0.19944
Rwork	0.197
R-free	0.24636
Structure solution method	MAD
Starting model (for MR)	NONE
RMSD bond length	0.016
RMSD bond angle	1.559
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	autoSHARP
Refinement software	REFMAC (5.5.0110)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.130	2.160
High resolution limit [Å]	2.050	2.050
Rmerge	0.160	0.770
Number of reflections	32252
<I/σ(I)>	7.8	2
Completeness [%]	99.7	98.2
Redundancy	6.7	4.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		7.5		0.1M CALCIUM CHLORIDE, 26% PEG MONOMETHYLETHER 350, 0.1M HEPES PH 7.5
1		7.5		0.1M CALCIUM CHLORIDE, 26% PEG MONOMETHYLETHER 350, 0.1M HEPES PH 7.5