2XVM
Crystal structure of the tellurite detoxification protein TehB from E. coli in complex with SAH
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I02 |
Synchrotron site | Diamond |
Beamline | I02 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-08-07 |
Detector | ADSC CCD |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 130.362, 55.644, 59.420 |
Unit cell angles | 90.00, 97.72, 90.00 |
Refinement procedure
Resolution | 28.260 - 1.480 |
R-factor | 0.17337 |
Rwork | 0.170 |
R-free | 0.22976 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2i6g |
RMSD bond length | 0.021 |
RMSD bond angle | 1.912 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | REFMAC (5.5.0106) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 28.200 | 1.560 |
High resolution limit [Å] | 1.480 | 1.480 |
Rmerge | 0.060 | 0.430 |
Number of reflections | 69781 | |
<I/σ(I)> | 16.1 | 2.7 |
Completeness [%] | 98.9 | 96.2 |
Redundancy | 5.3 | 2.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 8 | 0.2M NAF, 22% PEG3350, 1MM SAM, pH 8 |