2XVM
Crystal structure of the tellurite detoxification protein TehB from E. coli in complex with SAH
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-08-07 |
| Detector | ADSC CCD |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 130.362, 55.644, 59.420 |
| Unit cell angles | 90.00, 97.72, 90.00 |
Refinement procedure
| Resolution | 28.260 - 1.480 |
| R-factor | 0.17337 |
| Rwork | 0.170 |
| R-free | 0.22976 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2i6g |
| RMSD bond length | 0.021 |
| RMSD bond angle | 1.912 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0106) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.200 | 1.560 |
| High resolution limit [Å] | 1.480 | 1.480 |
| Rmerge | 0.060 | 0.430 |
| Number of reflections | 69781 | |
| <I/σ(I)> | 16.1 | 2.7 |
| Completeness [%] | 98.9 | 96.2 |
| Redundancy | 5.3 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8 | 0.2M NAF, 22% PEG3350, 1MM SAM, pH 8 |
