2XVF
Crystal structure of bacterial flavin-containing monooxygenase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 4A |
| Synchrotron site | PAL/PLS |
| Beamline | 4A |
| Temperature [K] | 294 |
| Detector technology | CCD |
| Collection date | 2006-04-05 |
| Detector | ADSC CCD |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 158.620, 70.085, 140.960 |
| Unit cell angles | 90.00, 90.71, 90.00 |
Refinement procedure
| Resolution | 34.380 - 2.400 |
| R-factor | 0.18628 |
| Rwork | 0.184 |
| R-free | 0.23570 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.137 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.5.0088) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.490 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.120 | 0.450 |
| Number of reflections | 60332 | |
| <I/σ(I)> | 20 | 3.64 |
| Completeness [%] | 98.4 | 92.2 |
| Redundancy | 6.5 | 5.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 10% PEG 3350, 0.2 M AMMONIUM NITRATE |
