2XV3
Pseudomonas aeruginosa Azurin with mutated metal-binding loop sequence (CAAAAHAAAAM), chemically reduced, pH5.3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Detector | RIGAKU IMAGE PLATE |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 34.430, 86.593, 41.704 |
| Unit cell angles | 90.00, 114.43, 90.00 |
Refinement procedure
| Resolution | 29.480 - 2.300 |
| R-factor | 0.18717 |
| Rwork | 0.183 |
| R-free | 0.26594 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3fsz |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.471 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Refinement software | REFMAC (5.3.0034) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.490 | 2.420 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.100 | 0.350 |
| Number of reflections | 9493 | |
| <I/σ(I)> | 18.9 | 5 |
| Completeness [%] | 95.9 | 92.1 |
| Redundancy | 6.4 | 6.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.3 | 293 | 10MM TRI-SODIUM CITRATE, 33% PEG6000, PH 7.6, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 293K |
