2XUZ
Crystal structure of the triscatecholate siderophore binding protein FeuA from Bacillus subtilis complexed with Ferri-Enterobactin
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-2 |
Synchrotron site | ESRF |
Beamline | ID14-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-03-08 |
Detector | ADSC CCD |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 36.440, 63.560, 56.520 |
Unit cell angles | 90.00, 100.65, 90.00 |
Refinement procedure
Resolution | 41.830 - 1.900 |
R-factor | 0.18974 |
Rwork | 0.185 |
R-free | 0.24320 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2why |
RMSD bond length | 0.009 |
RMSD bond angle | 1.213 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 41.830 | 2.000 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.060 | 0.490 |
Number of reflections | 19907 | |
<I/σ(I)> | 14.8 | 2.5 |
Completeness [%] | 99.1 | 97.7 |
Redundancy | 3.1 | 3.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 5.2 | PROTEIN WAS CRYSTALLIZED FROM 34% (V/V) PEG 600, 100 MM PHOSPHATE-CITRATE, PH 5.20 |