2XUT
Crystal structure of a proton dependent oligopeptide (POT) family transporter.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I02 |
Synchrotron site | Diamond |
Beamline | I02 |
Temperature [K] | 277 |
Detector technology | CCD |
Collection date | 2009-02-18 |
Detector | ADSC CCD |
Spacegroup name | P 32 |
Unit cell lengths | 159.400, 159.400, 152.940 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 35.470 - 3.620 |
R-factor | 0.2776 |
Rwork | 0.277 |
R-free | 0.29630 |
Structure solution method | MIRAS |
RMSD bond length | 0.009 |
RMSD bond angle | 1.120 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SHARP |
Refinement software | BUSTER (2.8.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 35.500 | 3.730 |
High resolution limit [Å] | 3.620 | 3.600 |
Rmerge | 0.090 | 0.680 |
Number of reflections | 47021 | |
<I/σ(I)> | 9 | 1.1 |
Completeness [%] | 93.8 | 88.7 |
Redundancy | 2 | 1.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 6.5 | 30% PEG 300, 0.1M MES PH 6.50 AND 0.1M NACL |