2XUT
Crystal structure of a proton dependent oligopeptide (POT) family transporter.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 277 |
| Detector technology | CCD |
| Collection date | 2009-02-18 |
| Detector | ADSC CCD |
| Spacegroup name | P 32 |
| Unit cell lengths | 159.400, 159.400, 152.940 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 35.470 - 3.620 |
| R-factor | 0.2776 |
| Rwork | 0.277 |
| R-free | 0.29630 |
| Structure solution method | MIRAS |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.120 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHARP |
| Refinement software | BUSTER (2.8.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.500 | 3.730 |
| High resolution limit [Å] | 3.620 | 3.600 |
| Rmerge | 0.090 | 0.680 |
| Number of reflections | 47021 | |
| <I/σ(I)> | 9 | 1.1 |
| Completeness [%] | 93.8 | 88.7 |
| Redundancy | 2 | 1.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 30% PEG 300, 0.1M MES PH 6.50 AND 0.1M NACL |
