2XUS
Crystal Structure of the BRMS1 N-terminal region
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-11-20 |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 60.399, 60.399, 133.485 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 48.701 - 1.912 |
| R-factor | 0.204 |
| Rwork | 0.200 |
| R-free | 0.27540 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2ccf |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.478 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.700 | 2.020 |
| High resolution limit [Å] | 1.910 | 1.910 |
| Rmerge | 0.040 | 0.280 |
| Number of reflections | 7517 | |
| <I/σ(I)> | 79.3 | 14.5 |
| Completeness [%] | 99.7 | 100 |
| Redundancy | 40.4 | 30.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.25 | 0.1 M SODIUM CITRATE PH 5.25, 1.5 M AMMONIUM SULPHATE |
