2XUR
The G157C mutation in the Escherichia coli sliding clamp specifically affects initiation of replication
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 79.981, 66.487, 81.699 |
| Unit cell angles | 90.00, 113.75, 90.00 |
Refinement procedure
| Resolution | 36.610 - 1.900 |
| R-factor | 0.19673 |
| Rwork | 0.194 |
| R-free | 0.25180 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2pol |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.841 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.000 | 2.000 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Number of reflections | 61943 | |
| <I/σ(I)> | 9.2 | 2.2 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 3.1 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 |
