2XU7
Structural basis for RbAp48 binding to FOG-1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-06-29 |
| Detector | ADSC CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 75.745, 59.843, 100.648 |
| Unit cell angles | 90.00, 93.55, 90.00 |
Refinement procedure
| Resolution | 100.500 - 1.900 |
| R-factor | 0.18527 |
| Rwork | 0.183 |
| R-free | 0.22496 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3gfc |
| RMSD bond length | 0.023 |
| RMSD bond angle | 1.860 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 75.590 | 2.000 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.080 | 0.450 |
| Number of reflections | 70736 | |
| <I/σ(I)> | 11.3 | 3.1 |
| Completeness [%] | 100.0 | 99.9 |
| Redundancy | 3.2 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 20% (W/V) PEG 3350, 0.2 M NA-MALONATE PH 7.0 |
