2XTR
Structure of the P176A Colicin M mutant from E. coli
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 52.832, 114.772, 225.402 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.940 - 2.140 |
| R-factor | 0.19069 |
| Rwork | 0.188 |
| R-free | 0.24104 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2xmx |
| RMSD bond length | 0.023 |
| RMSD bond angle | 2.027 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.000 | 2.190 |
| High resolution limit [Å] | 2.140 | 2.140 |
| Rmerge | 0.190 | 0.820 |
| Number of reflections | 36063 | |
| <I/σ(I)> | 9.2 | 2 |
| Completeness [%] | 99.0 | 94.2 |
| Redundancy | 6.3 | 4.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 20% PEG3350 0.2 M NANO3, PH 6.5 |
