2XTP
Crystal structure of nucleotide-free human GIMAP2, amino acid residues 1-260
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.2 |
| Synchrotron site | BESSY |
| Beamline | 14.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-01-23 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.9797, 0.9849 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 57.380, 60.910, 72.020 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.140 - 1.500 |
| R-factor | 0.18128 |
| Rwork | 0.180 |
| R-free | 0.20382 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.161 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | autoSHARP |
| Refinement software | REFMAC (5.5.0070) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.800 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Rmerge | 0.070 | 0.500 |
| Number of reflections | 183804 | |
| <I/σ(I)> | 14.6 | 3.2 |
| Completeness [%] | 99.1 | 95.9 |
| Redundancy | 3.8 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 11-12% 2-PROPANOL, 15% GLYCEROL, pH 7.5 |
