2XTO
Crystal structure of GDP-bound human GIMAP2, amino acid residues 21- 260
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.2 |
Synchrotron site | BESSY |
Beamline | 14.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-05-02 |
Detector | MARRESEARCH |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 62.680, 76.550, 101.620 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 12.000 - 2.800 |
R-factor | 0.23176 |
Rwork | 0.229 |
R-free | 0.27565 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | NONE |
RMSD bond length | 0.006 |
RMSD bond angle | 0.970 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | MOLREP |
Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.970 |
High resolution limit [Å] | 2.800 | 2.800 |
Rmerge | 0.220 | 0.600 |
Number of reflections | 12565 | |
<I/σ(I)> | 8.5 | 2.89 |
Completeness [%] | 98.2 | 97.6 |
Redundancy | 4.1 | 3.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7.5 | 19% 2-PROPANOL, pH 7.5 |