2XTL
Structure of the major pilus backbone protein from Streptococcus Agalactiae
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-1 |
Synchrotron site | ESRF |
Beamline | ID23-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-06-25 |
Detector | ADSC CCD |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 63.863, 104.681, 159.250 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 40.000 - 1.750 |
R-factor | 0.1864 |
Rwork | 0.185 |
R-free | 0.21588 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2x9w |
RMSD bond length | 0.007 |
RMSD bond angle | 1.048 |
Data reduction software | iMOSFLM |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 1.840 |
High resolution limit [Å] | 1.750 | 1.750 |
Rmerge | 0.100 | 0.600 |
Number of reflections | 108305 | |
<I/σ(I)> | 14.9 | 3.7 |
Completeness [%] | 100.0 | 100 |
Redundancy | 9.6 | 9.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7.2 | 25% PEG4K, 0.1M HEPES PH 7.2, 90 MM POTASSIUM SODIUM TARTRATE |