2XTE
Structure of the TBL1 tetramerisation domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-06-22 |
| Detector | ADSC CCD |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 88.020, 150.190, 164.830 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 111.100 - 3.900 |
| R-factor | 0.273 |
| Rwork | 0.273 |
| R-free | 0.30200 |
| Structure solution method | MIR |
| Starting model (for MR) | 2xtc |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.240 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | CNS (1.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 111.100 | 4.110 |
| High resolution limit [Å] | 3.900 | 3.900 |
| Rmerge | 0.110 | 0.320 |
| Number of reflections | 19503 | |
| <I/σ(I)> | 9.1 | 4 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 3.5 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 0.1 M MES PH6.5, 2.0 M NACL, 0.175 M SODIUM ACETATE, 19 % GLYCEROL |
