2XTD
Structure of the TBL1 tetramerisation domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-08-26 |
| Detector | ADSC QUANTUM 4 |
| Spacegroup name | P 32 1 2 |
| Unit cell lengths | 47.886, 47.886, 153.811 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 51.300 - 3.200 |
| R-factor | 0.28001 |
| Rwork | 0.278 |
| R-free | 0.31530 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2xtc |
| RMSD bond length | 0.029 |
| RMSD bond angle | 2.002 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 51.000 | 3.370 |
| High resolution limit [Å] | 3.200 | 3.200 |
| Rmerge | 0.060 | 0.430 |
| Number of reflections | 3503 | |
| <I/σ(I)> | 20.2 | 5.1 |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 6.7 | 6.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 4.5 | 15% MPD, 0.05 M SODIUM ACETATE PH 4.5 |
