2XTC
Structure of the TBL1 tetramerisation domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-12-13 |
| Detector | ADSC CCD |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 59.541, 59.541, 193.880 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 51.570 - 2.220 |
| R-factor | 0.23854 |
| Rwork | 0.237 |
| R-free | 0.26437 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1uuj |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.494 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 96.000 | 2.320 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.070 | 0.400 |
| Number of reflections | 10905 | |
| <I/σ(I)> | 15.1 | 3.9 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 6.5 | 6.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.4 | 17% PEG 4000, 0.2 M MGCL2, 0.1 M TRIS/HCL PH8.5, pH 7.4 |
