2XTA
Crystal structure of the SucA domain of Mycobacterium smegmatis alpha- ketoglutarate decarboxylase in complex with acetyl-CoA (triclinic form)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-11-21 |
| Detector | MARMOSAIC 225 mm CCD |
| Spacegroup name | P 1 |
| Unit cell lengths | 80.550, 83.580, 160.070 |
| Unit cell angles | 99.59, 98.94, 100.68 |
Refinement procedure
| Resolution | 78.110 - 2.200 |
| R-factor | 0.1892 |
| Rwork | 0.188 |
| R-free | 0.21360 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2XT9 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.020 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | BUSTER (2.9.3) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 78.100 | 2.310 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.070 | 0.400 |
| Number of reflections | 187791 | |
| <I/σ(I)> | 8.7 | 2.2 |
| Completeness [%] | 92.6 | 88.5 |
| Redundancy | 2 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.6 | 54% MPD, 25 MM SODIUM ACETATE, pH 7.6 |
