2XT4
Structure of the pentapeptide repeat protein AlbG, a resistance factor for the topoisomerase poison albicidin.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 93 |
| Detector technology | IMAGE PLATE |
| Detector | RIGAKU RAXIS IV |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 58.532, 65.623, 105.917 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 25.179 - 2.394 |
| R-factor | 0.2082 |
| Rwork | 0.204 |
| R-free | 0.29280 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2xt2 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.364 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.520 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.040 | 0.310 |
| Number of reflections | 16626 | |
| <I/σ(I)> | 15.7 | 3.3 |
| Completeness [%] | 99.4 | 96 |
| Redundancy | 4.3 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | COMBINING PROTEIN, 2 UL OF 15 MG ML-1, 20 MM TRIS PH 8.0, 50 MM NACL, 1 MM DTT, 1MM EDTA WITH PRECIPITANT, 100 MM AMMONIUM TARTRATE PH 7.0, 12% PEG 3350 |
