2XT0
Dehalogenase DPpA from Plesiocystis pacifica SIR-I
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-05-04 |
| Detector | RIGAKU SATURN 92 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 47.262, 108.501, 67.312 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 67.310 - 1.900 |
| R-factor | 0.22081 |
| Rwork | 0.219 |
| R-free | 0.24801 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1edb |
| RMSD bond length | 0.023 |
| RMSD bond angle | 1.854 |
| Data reduction software | CrystalClear (V.1.3.6) |
| Data scaling software | CrystalClear (V.1.3.6) |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.680 | 2.020 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.120 | 0.440 |
| Number of reflections | 25613 | |
| <I/σ(I)> | 7 | 2.3 |
| Completeness [%] | 98.5 | 97.4 |
| Redundancy | 5.5 | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 0.1 M MES PH 6.5, 1.8M (NH4)2SO4, 5% PEG400 |
