2XSF
Crystal structure of the RRM domain of mouse Deleted in azoospermia- like
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SRS BEAMLINE PX14.2 |
Synchrotron site | SRS |
Beamline | PX14.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-09-18 |
Detector | ADSC CCD |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 53.900, 53.900, 65.930 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 22.000 - 1.700 |
R-factor | 0.189 |
Rwork | 0.189 |
R-free | 0.20200 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3bs9 |
RMSD bond length | 0.014 |
RMSD bond angle | 1.587 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 1.790 |
High resolution limit [Å] | 1.700 | 1.700 |
Rmerge | 0.070 | 0.600 |
Number of reflections | 12616 | |
<I/σ(I)> | 21.9 | 4.7 |
Completeness [%] | 99.9 | 100 |
Redundancy | 11.8 | 12 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7.5 | 2.1 M LITHIUM SULPHATE, 50 MM HEPES PH 7.5, 5 % (V/V) GLYCEROL |