2XS7
Crystal structure of the RRM domain of mouse Deleted in azoospermia- like in complex with Sycp3 RNA, UUGUUU
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-05-16 |
| Detector | ADSC CCD |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 32.888, 37.749, 70.771 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.830 - 1.450 |
| R-factor | 0.16471 |
| Rwork | 0.162 |
| R-free | 0.20469 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2xs2 |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.615 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.820 | 1.530 |
| High resolution limit [Å] | 1.450 | 1.450 |
| Rmerge | 0.080 | 0.920 |
| Number of reflections | 16096 | |
| <I/σ(I)> | 16.1 | 2.1 |
| Completeness [%] | 99.1 | 97.7 |
| Redundancy | 6.9 | 6.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 10 MM MAGNESIUM FORMATE, 25% (W/V) PEG 3350 10 MM ZINC ACETATE |
