2XS5
Crystal structure of the RRM domain of mouse Deleted in azoospermia- like in complex with Mvh RNA, UGUUC
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-05-15 |
| Detector | ADSC CCD |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 75.605, 77.538, 37.695 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 27.070 - 1.600 |
| R-factor | 0.19033 |
| Rwork | 0.188 |
| R-free | 0.22418 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2xs2 |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.511 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 27.070 | 1.690 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.090 | 0.760 |
| Number of reflections | 29634 | |
| <I/σ(I)> | 11.2 | 2.1 |
| Completeness [%] | 98.7 | 98 |
| Redundancy | 4.9 | 4.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 0.1 M MAGNESIUM ACETATE, 18% (W/V) PEG 3350 |
