2XS1
Crystal Structure of ALIX in complex with the SIVmac239 PYKEVTEDL Late Domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-09-23 |
| Detector | ADSC CCD |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 145.281, 99.271, 72.521 |
| Unit cell angles | 90.00, 106.62, 90.00 |
Refinement procedure
| Resolution | 35.440 - 2.296 |
| R-factor | 0.2075 |
| Rwork | 0.205 |
| R-free | 0.25230 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2oev |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.994 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | REFMAC |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.000 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.100 | 0.280 |
| Number of reflections | 44063 | |
| <I/σ(I)> | 18.2 | 3.9 |
| Completeness [%] | 97.7 | 82.6 |
| Redundancy | 7 | 5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.2 | 6.5% PEG4000, 0.2 MGCL2, 0.1M MES PH 6.2 |
