2XRB
Structure of the N-terminal four domains of the complement regulator Rat Crry
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 120 |
| Detector technology | CCD |
| Collection date | 2010-07-11 |
| Detector | ADSC CCD |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 21.770, 105.339, 152.281 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.260 - 2.500 |
| R-factor | 0.1872 |
| Rwork | 0.185 |
| R-free | 0.23700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | DOMAINS FROM PDB ENTRIES 1OK9 AND 1GKN |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.170 |
| Data reduction software | xia2 (USING XDS) |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.9.5) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.470 |
| High resolution limit [Å] | 2.500 | 2.410 |
| Rmerge | 0.120 | 0.640 |
| Number of reflections | 13105 | |
| <I/σ(I)> | 12.8 | 2.5 |
| Completeness [%] | 99.8 | 97.9 |
| Redundancy | 6.6 | 5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 4.6 | 0.2 M AMMONIUM SULPHATE, 0.1 M SODIUM ACETATE PH 4.6, 30% W/V PEGMME 2000. |
