2XRB
Structure of the N-terminal four domains of the complement regulator Rat Crry
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04 |
Synchrotron site | Diamond |
Beamline | I04 |
Temperature [K] | 120 |
Detector technology | CCD |
Collection date | 2010-07-11 |
Detector | ADSC CCD |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 21.770, 105.339, 152.281 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.260 - 2.500 |
R-factor | 0.1872 |
Rwork | 0.185 |
R-free | 0.23700 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | DOMAINS FROM PDB ENTRIES 1OK9 AND 1GKN |
RMSD bond length | 0.010 |
RMSD bond angle | 1.170 |
Data reduction software | xia2 (USING XDS) |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | BUSTER (2.9.5) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.470 |
High resolution limit [Å] | 2.500 | 2.410 |
Rmerge | 0.120 | 0.640 |
Number of reflections | 13105 | |
<I/σ(I)> | 12.8 | 2.5 |
Completeness [%] | 99.8 | 97.9 |
Redundancy | 6.6 | 5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 4.6 | 0.2 M AMMONIUM SULPHATE, 0.1 M SODIUM ACETATE PH 4.6, 30% W/V PEGMME 2000. |