2XR8
Crystal structure of biphenyl dioxygenase from Burkholderia xenovorans LB400
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 14-ID-B |
| Synchrotron site | APS |
| Beamline | 14-ID-B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-07-25 |
| Detector | ADSC SX-165 |
| Spacegroup name | P 1 |
| Unit cell lengths | 132.585, 132.350, 132.984 |
| Unit cell angles | 102.60, 102.68, 104.61 |
Refinement procedure
| Resolution | 129.100 - 2.490 |
| R-factor | 0.21649 |
| Rwork | 0.216 |
| R-free | 0.26714 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3gzy |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.925 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 120.000 | 2.600 |
| High resolution limit [Å] | 2.490 | 2.490 |
| Rmerge | 0.100 | 0.410 |
| Number of reflections | 255418 | |
| <I/σ(I)> | 6.8 | 1.4 |
| Completeness [%] | 89.0 | 30 |
| Redundancy | 2.8 | 1.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6 | pH 6 |
