2XQU
Microscopic rotary mechanism of ion translocation in the Fo complex of ATP synthases
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-09-03 |
| Detector | ADSC CCD |
| Spacegroup name | P 63 2 2 |
| Unit cell lengths | 93.890, 93.890, 257.320 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 43.486 - 1.840 |
| R-factor | 0.1909 |
| Rwork | 0.189 |
| R-free | 0.22350 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2wie |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.892 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.000 |
| High resolution limit [Å] | 1.840 | 1.840 |
| Rmerge | 0.200 | 1.320 |
| Number of reflections | 58617 | |
| <I/σ(I)> | 13.5 | 1.8 |
| Completeness [%] | 99.1 | 97.2 |
| Redundancy | 12.9 | 10.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 4.3 | pH 4.3 |
