2XQT
Microscopic rotary mechanism of ion translocation in the Fo complex of ATP synthases
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2009-09-26 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 63 2 2 |
| Unit cell lengths | 91.470, 91.470, 258.120 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 45.030 - 2.200 |
| R-factor | 0.188 |
| Rwork | 0.186 |
| R-free | 0.22300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2wie |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.494 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.000 | 2.300 |
| High resolution limit [Å] | 2.170 | 2.170 |
| Rmerge | 0.150 | 0.770 |
| Number of reflections | 34801 | |
| <I/σ(I)> | 12.02 | 3.03 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 14.3 | 14.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 4.3 | PH 4.3 |
