2XQN
Complex of the 2nd and 3rd LIM domains of TES with the EVH1 DOMAIN of MENA and the N-Terminal domain of actin-like protein Arp7A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2007-08-17 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 37.829, 86.791, 115.266 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 25.560 - 2.620 |
| R-factor | 0.21776 |
| Rwork | 0.215 |
| R-free | 0.26811 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2iyb |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.894 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.740 | 2.740 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.110 | 0.500 |
| Number of reflections | 12008 | |
| <I/σ(I)> | 14.2 | 2.9 |
| Completeness [%] | 96.9 | 79.7 |
| Redundancy | 4.2 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 295 | PROTEIN: 37.5 MG/ML OF PROTEIN COMPLEX IN 20MM TRIS-HCL PH 8.0, 100MM NACL RESERVOIR: 17.5 PEG 8K, 0.1MTRIS PH 8.5, 0.15M KSCN SITTING DROPS 2:1 PROTEIN:RESERVOIR, 22 DEG C CRYOPROTECTANT: N-PARATONE |
