2XQL
Fitting of the H2A-H2B histones in the electron microscopy map of the complex Nucleoplasmin:H2A-H2B histones (1:5).
EM specimen
| Staining | Yes |
| Staining material | Uranyl Acetate |
EM Data Collection
| Microscope | JEOL 1200EXII |
| Electron Gun | TUNGSTEN HAIRPIN |
| Accelerating Voltage (kV) | 100 |
| Cs (mm) | 5.6 |
| Detector | KODAK SO-163 FILM |
| Number of Images | 14 |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 19.5 |
| Software | Situs,EMAN,SPIDER,Xmipp |
| Symmetry | C5 |
