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2XPN

Crystal structure of a Spt6-Iws1(Spn1) complex from Encephalitozoon cuniculi, Form I

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsESRF BEAMLINE ID29
Synchrotron siteESRF
BeamlineID29
Temperature [K]100
Detector technologyCCD
Collection date2008-11-29
DetectorADSC CCD
Spacegroup nameP 41 21 2
Unit cell lengths52.311, 52.311, 151.125
Unit cell angles90.00, 90.00, 90.00
Refinement procedure
Resolution49.450 - 1.950
R-factor0.21112
Rwork0.210
R-free0.23486
Structure solution methodMOLECULAR REPLACEMENT
Starting model (for MR)2xpl
RMSD bond length0.016
RMSD bond angle1.494
Data reduction softwareDENZO
Data scaling softwareSCALEPACK
Phasing softwarePHASER
Refinement softwareREFMAC (5.5.0109)
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]50.0002.020
High resolution limit [Å]1.9501.950
Rmerge0.0400.170
Number of reflections16077
<I/σ(I)>78.817
Completeness [%]99.499.6
Redundancy12.411.4
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
122% PEG3350, 0.2 M NABR

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