2XPN
Crystal structure of a Spt6-Iws1(Spn1) complex from Encephalitozoon cuniculi, Form I
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID29 |
Synchrotron site | ESRF |
Beamline | ID29 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-11-29 |
Detector | ADSC CCD |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 52.311, 52.311, 151.125 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.450 - 1.950 |
R-factor | 0.21112 |
Rwork | 0.210 |
R-free | 0.23486 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2xpl |
RMSD bond length | 0.016 |
RMSD bond angle | 1.494 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.020 |
High resolution limit [Å] | 1.950 | 1.950 |
Rmerge | 0.040 | 0.170 |
Number of reflections | 16077 | |
<I/σ(I)> | 78.8 | 17 |
Completeness [%] | 99.4 | 99.6 |
Redundancy | 12.4 | 11.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 22% PEG3350, 0.2 M NABR |