2XPL
Crystal structure of Iws1(Spn1) conserved domain from Encephalitozoon cuniculi
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-2 |
Synchrotron site | ESRF |
Beamline | ID14-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-09-20 |
Detector | ADSC CCD |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 33.655, 128.832, 33.644 |
Unit cell angles | 90.00, 101.17, 90.00 |
Refinement procedure
Resolution | 64.420 - 2.250 |
R-factor | 0.20604 |
Rwork | 0.203 |
R-free | 0.26413 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | MAD STRUCTURE - UNPUBLISHED |
RMSD bond length | 0.019 |
RMSD bond angle | 1.781 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 64.420 | 2.320 |
High resolution limit [Å] | 2.250 | 2.250 |
Rmerge | 0.060 | 0.120 |
Number of reflections | 13209 | |
<I/σ(I)> | 25.9 | 15.1 |
Completeness [%] | 99.2 | 99.3 |
Redundancy | 4.6 | 4.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7 | 0.1 M 2-(N-MORPHOLINO)ETHANESULFONIC ACID PH 7.0, 20% PEG8000, 5% ETHYL ACETATE |