2XPL
Crystal structure of Iws1(Spn1) conserved domain from Encephalitozoon cuniculi
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-09-20 |
| Detector | ADSC CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 33.655, 128.832, 33.644 |
| Unit cell angles | 90.00, 101.17, 90.00 |
Refinement procedure
| Resolution | 64.420 - 2.250 |
| R-factor | 0.20604 |
| Rwork | 0.203 |
| R-free | 0.26413 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | MAD STRUCTURE - UNPUBLISHED |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.781 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 64.420 | 2.320 |
| High resolution limit [Å] | 2.250 | 2.250 |
| Rmerge | 0.060 | 0.120 |
| Number of reflections | 13209 | |
| <I/σ(I)> | 25.9 | 15.1 |
| Completeness [%] | 99.2 | 99.3 |
| Redundancy | 4.6 | 4.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 0.1 M 2-(N-MORPHOLINO)ETHANESULFONIC ACID PH 7.0, 20% PEG8000, 5% ETHYL ACETATE |
