2XP0
C-terminal cysteine-rich domain of human CHFR
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC CCD |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 163.010, 52.030, 82.520 |
| Unit cell angles | 90.00, 105.73, 90.00 |
Refinement procedure
| Resolution | 39.715 - 1.978 |
| R-factor | 0.1642 |
| Rwork | 0.161 |
| R-free | 0.20200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2xoc |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.028 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.510 | 2.090 |
| High resolution limit [Å] | 1.980 | 1.980 |
| Rmerge | 0.100 | 0.460 |
| Number of reflections | 46604 | |
| <I/σ(I)> | 9.1 | 2.6 |
| Completeness [%] | 99.5 | 99.8 |
| Redundancy | 3.5 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 12% PEG 20000, 0.1M MES PH 6.5, 0.1M KCL |
