2XOT
Crystal structure of neuronal leucine rich repeat protein AMIGO-1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-10-27 |
| Detector | ADSC CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 61.440, 74.630, 87.270 |
| Unit cell angles | 90.00, 98.66, 90.00 |
Refinement procedure
| Resolution | 29.100 - 2.000 |
| R-factor | 0.2102 |
| Rwork | 0.208 |
| R-free | 0.25541 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.417 |
| Data scaling software | XDS |
| Phasing software | SHELX |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.100 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.090 | 0.750 |
| Number of reflections | 52608 | |
| <I/σ(I)> | 9.5 | 1.7 |
| Completeness [%] | 99.2 | 98.4 |
| Redundancy | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 1.6 M MGSO4, pH 6.5 |
