2XON
Structure of TmCBM61 in complex with beta-1,4-galactotriose at 1.4 A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-2 |
| Synchrotron site | SSRL |
| Beamline | BL9-2 |
| Temperature [K] | 113 |
| Detector technology | CCD |
| Detector | ADSC CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 48.900, 61.850, 55.530 |
| Unit cell angles | 90.00, 105.14, 90.00 |
Refinement procedure
| Resolution | 34.260 - 1.400 |
| R-factor | 0.11904 |
| Rwork | 0.116 |
| R-free | 0.16673 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2xom |
| RMSD bond length | 0.024 |
| RMSD bond angle | 2.184 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.480 |
| High resolution limit [Å] | 1.400 | 1.400 |
| Rmerge | 0.040 | 0.180 |
| Number of reflections | 56801 | |
| <I/σ(I)> | 20.4 | 7.6 |
| Completeness [%] | 95.3 | 77.9 |
| Redundancy | 5.7 | 5.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 |
