2XOC
C-terminal cysteine-rich domain of human CHFR bound to mADPr
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC CCD |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 161.540, 51.700, 82.360 |
| Unit cell angles | 90.00, 105.76, 90.00 |
Refinement procedure
| Resolution | 32.513 - 1.890 |
| R-factor | 0.1702 |
| Rwork | 0.168 |
| R-free | 0.19350 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.845 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.220 | 1.990 |
| High resolution limit [Å] | 1.890 | 1.890 |
| Rmerge | 0.090 | 0.450 |
| Number of reflections | 52431 | |
| <I/σ(I)> | 8.7 | 2.5 |
| Completeness [%] | 99.5 | 99.9 |
| Redundancy | 3.3 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 12% PEG 20000, 0.1M MES PH 6.5 0.1M KCL |
