2XOA
Crystal Structure of the N-terminal three domains of the skeletal muscle Ryanodine Receptor (RyR1)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CLSI BEAMLINE 08ID-1 |
Synchrotron site | CLSI |
Beamline | 08ID-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-03-08 |
Detector | MARRESEARCH |
Spacegroup name | H 3 2 |
Unit cell lengths | 170.800, 170.800, 301.200 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 49.450 - 2.500 |
R-factor | 0.21032 |
Rwork | 0.209 |
R-free | 0.23373 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.018 |
RMSD bond angle | 1.788 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.640 |
High resolution limit [Å] | 2.500 | 2.500 |
Rmerge | 0.110 | 0.600 |
Number of reflections | 58314 | |
<I/σ(I)> | 15.6 | 2.5 |
Completeness [%] | 92.9 | 81.4 |
Redundancy | 11.3 | 11.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 8 | pH 8 |