2XO3
Human Annexin V with incorporated Methionine analogue Homopropargylglycine
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | MPG/DESY, HAMBURG BEAMLINE BW6 |
Synchrotron site | MPG/DESY, HAMBURG |
Beamline | BW6 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-12-23 |
Detector | MARRESEARCH |
Spacegroup name | H 3 |
Unit cell lengths | 154.965, 154.965, 35.580 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 19.960 - 2.300 |
R-factor | 0.262 |
Rwork | 0.262 |
R-free | 0.29100 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1anx |
RMSD bond length | 0.007 |
RMSD bond angle | 1.300 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | CNS (1.3) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.420 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.070 | 0.230 |
Number of reflections | 14143 | |
<I/σ(I)> | 24.8 | 11.2 |
Completeness [%] | 99.9 | 100 |
Redundancy | 8.1 | 8.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 8.5 | 2.0 M AMMONIUM SULFATE, 20 MM CALCIUM CHLORIDE, 0.1 M TRIS/HCL, PH 8.5 |