2XO1
xpt-pbuX C74U Riboswitch from B. subtilis bound to N6-methyladenine
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I03 |
Synchrotron site | Diamond |
Beamline | I03 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-05-23 |
Detector | ADSC CCD |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 132.762, 35.180, 41.736 |
Unit cell angles | 90.00, 90.51, 90.00 |
Refinement procedure
Resolution | 66.380 - 1.600 |
R-factor | 0.19554 |
Rwork | 0.195 |
R-free | 0.21075 |
Structure solution method | OTHER |
Starting model (for MR) | NONE |
RMSD bond length | 0.008 |
RMSD bond angle | 1.508 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 66.380 |
High resolution limit [Å] | 1.600 |
Rmerge | 0.050 |
Number of reflections | 25098 |
<I/σ(I)> | 20.6 |
Completeness [%] | 96.5 |
Redundancy | 2.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7.5 | 10MM K-HEPES PH 7.5, 20% PEG 3000, 12MM COHEX, 440MM AMMONIUM ACETATE |