2XO1
xpt-pbuX C74U Riboswitch from B. subtilis bound to N6-methyladenine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-05-23 |
| Detector | ADSC CCD |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 132.762, 35.180, 41.736 |
| Unit cell angles | 90.00, 90.51, 90.00 |
Refinement procedure
| Resolution | 66.380 - 1.600 |
| R-factor | 0.19554 |
| Rwork | 0.195 |
| R-free | 0.21075 |
| Structure solution method | OTHER |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.508 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 66.380 |
| High resolution limit [Å] | 1.600 |
| Rmerge | 0.050 |
| Number of reflections | 25098 |
| <I/σ(I)> | 20.6 |
| Completeness [%] | 96.5 |
| Redundancy | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 10MM K-HEPES PH 7.5, 20% PEG 3000, 12MM COHEX, 440MM AMMONIUM ACETATE |
