2XNQ
Structural insights into cis element recognition of non- polyadenylated RNAs by the Nab3-RRM
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2010-02-18 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 26.950, 42.070, 55.670 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 33.560 - 1.300 |
| R-factor | 0.17567 |
| Rwork | 0.175 |
| R-free | 0.19448 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1wf2 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.335 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.400 |
| High resolution limit [Å] | 1.300 | 1.300 |
| Rmerge | 0.060 | 0.210 |
| Number of reflections | 15337 | |
| <I/σ(I)> | 19.8 | 7.54 |
| Completeness [%] | 98.3 | 95 |
| Redundancy | 6 | 5.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 100MM SODIUM CACODYLATE BUFFER PH 6.5, 200MM SODIUM ACETATE, PEG 8000 AT 20-30 % (W/V). |
