2XND
Crystal structure of bovine F1-c8 sub-complex of ATP Synthase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-05-02 |
Detector | MARRESEARCH |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 155.740, 157.100, 247.270 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 132.600 - 3.500 |
R-factor | 0.26341 |
Rwork | 0.261 |
R-free | 0.30397 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1e79 |
RMSD bond length | 0.006 |
RMSD bond angle | 0.996 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 132.600 | 3.590 |
High resolution limit [Å] | 3.500 | 3.500 |
Rmerge | 0.260 | 0.820 |
Number of reflections | 72959 | |
<I/σ(I)> | 3.7 | 1.6 |
Completeness [%] | 99.6 | 99.5 |
Redundancy | 3.6 | 3.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7 | CRYSTALS WERE GROWN UNDER OIL BY MIXING EQUAL VOLUMES OF PROTEIN (10MG/ML IN 20MM TRIS PH 8.0, 10% GLYCEROL, 1MM ADP, 1MM AMP-PNP, 2MM MGSO4, 0.02% NAN3, 5.7MM TDM) AND PRECIPITANT SOLUTION (50MM HEPES PH 7.0, 14% PEG4600, 50MM K2HPO4) |