2XN4
Crystal structure of the kelch domain of human KLHL2 (Mayven)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2010-05-03 |
| Detector | DECTRIS PILATUS |
| Spacegroup name | P 1 |
| Unit cell lengths | 45.994, 46.005, 71.762 |
| Unit cell angles | 86.68, 82.75, 68.48 |
Refinement procedure
| Resolution | 19.820 - 1.990 |
| R-factor | 0.17195 |
| Rwork | 0.169 |
| R-free | 0.22155 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ade |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.587 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.830 | 2.090 |
| High resolution limit [Å] | 1.990 | 1.990 |
| Rmerge | 0.100 | 0.320 |
| Number of reflections | 34329 | |
| <I/σ(I)> | 8.9 | 4.3 |
| Completeness [%] | 92.5 | 67.8 |
| Redundancy | 3.1 | 2.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 0.2M (NH4)2SO4, 0.1M MES PH 6.5, 30% PEG 5000 MME, 0.2M NASCN |
