2XN0
Structure of alpha-galactosidase from Lactobacillus acidophilus NCFM, PtCl4 derivative
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | MAX II BEAMLINE I911-2 |
Synchrotron site | MAX II |
Beamline | I911-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-03-19 |
Detector | MARRESEARCH MAR165 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 126.370, 149.580, 90.840 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.839 - 2.500 |
R-factor | 0.1502 |
Rwork | 0.148 |
R-free | 0.20260 |
Structure solution method | SAD |
Starting model (for MR) | NONE |
RMSD bond length | 0.007 |
RMSD bond angle | 1.072 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHENIX |
Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.560 |
High resolution limit [Å] | 2.500 | 2.500 |
Rmerge | 0.130 | 0.500 |
Number of reflections | 114981 | |
<I/σ(I)> | 15.2 | 4.9 |
Completeness [%] | 98.4 | 96.4 |
Redundancy | 7.6 | 7.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7.5 | 8-12% PEG8000, 0.1M IMIDAZOLE, 10% GLYCEROL., PH 7.5 |