2XMZ
Structure of MenH from S. aureus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2008-09-10 |
| Detector | RIGAKU IMAGE PLATE |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 76.548, 43.563, 71.650 |
| Unit cell angles | 90.00, 98.26, 90.00 |
Refinement procedure
| Resolution | 19.730 - 1.940 |
| R-factor | 0.18642 |
| Rwork | 0.183 |
| R-free | 0.24073 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1r3d |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.253 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.730 | 2.050 |
| High resolution limit [Å] | 1.940 | 1.940 |
| Rmerge | 0.050 | 0.370 |
| Number of reflections | 17242 | |
| <I/σ(I)> | 17.5 | 3.2 |
| Completeness [%] | 98.9 | 93.3 |
| Redundancy | 4.1 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | PROTEIN BUFFER: 20 MM TRIS-HCL, 50 MM NACL, PH 7.5. RESERVOIR: 0.1 M NA HEPES PH 7.5, 25 % W/V PEG 4000. |
