2XMX
High resolution structure of Colicin M
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC CCD |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 52.757, 115.007, 227.714 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.920 - 1.670 |
| R-factor | 0.15955 |
| Rwork | 0.157 |
| R-free | 0.19504 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3da4 |
| RMSD bond length | 0.027 |
| RMSD bond angle | 2.079 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.000 | 1.710 |
| High resolution limit [Å] | 1.670 | 1.670 |
| Rmerge | 0.080 | 0.570 |
| Number of reflections | 73413 | |
| <I/σ(I)> | 14.2 | 1.9 |
| Completeness [%] | 97.3 | 83.9 |
| Redundancy | 5.2 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 20% PEG3350, 0.2M NANO3 |
