2XMV
Copper chaperone Atx1 from Synechocystis PCC6803 (Cu1, trimeric form, His61Tyr mutant)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC CCD |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 47.690, 77.100, 90.500 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.970 - 1.800 |
| R-factor | 0.19372 |
| Rwork | 0.191 |
| R-free | 0.25315 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2xmj |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.466 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.900 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.110 | 0.360 |
| Number of reflections | 31381 | |
| <I/σ(I)> | 29.7 | 13.1 |
| Completeness [%] | 99.3 | 99.5 |
| Redundancy | 4.8 | 4.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 2.1 M D,L-MALIC ACID AT PH 7.0 |
