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Crystal structure of human JMJD2C catalytic domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-05-31 |
| Detector | MARRESEARCH |
| Spacegroup name | P 2 21 21 |
| Unit cell lengths | 88.660, 95.870, 100.690 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.572 - 2.550 |
| R-factor | 0.1991 |
| Rwork | 0.196 |
| R-free | 0.25400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2oq7 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.202 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 88.660 | 2.690 |
| High resolution limit [Å] | 2.550 | 2.550 |
| Rmerge | 0.370 | 0.740 |
| Number of reflections | 28447 | |
| <I/σ(I)> | 7.3 | 2.4 |
| Completeness [%] | 99.1 | 96 |
| Redundancy | 9.7 | 5.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 0.2M NA_NITRATE; 15% PEG_3350; 5% V/V ETHYLENE_GLYCOL; 0.1M BIS_TRIS_PROPANE PH 6.2 |
