2XMK
Visualising the Metal-binding Versatility of Copper Trafficking Sites: Atx1 side-to-side (anaerobic)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC CCD |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 62.991, 41.709, 55.791 |
| Unit cell angles | 90.00, 107.49, 90.00 |
Refinement procedure
| Resolution | 53.220 - 1.350 |
| R-factor | 0.13055 |
| Rwork | 0.129 |
| R-free | 0.15274 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2xmj |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.442 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 53.220 | 1.420 |
| High resolution limit [Å] | 1.350 | 1.350 |
| Rmerge | 0.080 | 0.290 |
| Number of reflections | 29653 | |
| <I/σ(I)> | 15.4 | 5.1 |
| Completeness [%] | 97.4 | 95.9 |
| Redundancy | 4.9 | 4.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 24 % (W/V) PEG 8000, 200 MM SODIUM ACETATE, 100 MM SODIUM CACODYLATE PH 6.5 |
